Effect of sevoflurane delayed-postconditioning on ischemia-reperfusion injury in isolated rat hearts

Purpose: To evaluate the protective effect, effective time window, and mechanism of protection sevoflurane post-conditioning late on heart muscle ischemiareperfusion (I/R) lesion in isolated rat heart.Methods: Langendorff perfusion model was established Sprague-Dawley (SD) rats. After 15 min stabilization, rats were assigned to 10 groups, based treatments given: control group (TTC), I/R group, post-processing (SpostC), 0.5 min-, 1 5 20 30 min- delayed (S0.5, S1, S3, S5, S10, S20). The levels mitochondrial membrane permeability, area myocardial infarction, apoptosis, p-GSK3 beta level determined.Results: Myocardial infarction areas Spost, S0.5, S10 S20 groups significantly than (p < 0.05), while expression p-Akt higher S20, S30 0.05). GSK-3 phosphorylation Expression p-GSK-3β decreased, relative that Spost 0.05).Conclusion: Sevoflurane mitigates lesions hearts. effect against ischemia-reperfusion injury is related Akt GSK-3β. Thus, this treatment approach may be useful management cardiac injury.